Abstract:
Objetive: Culturing liquid hyphae with
Hericium erinaceus pure strain. The medium was optimized to increase the content of
Hericium erinaceus mycelium phenolic extracts. The composition of
Hericium erinaceus mycelium phenolic extracts was analyzed, and the effects of phenolic extracts from
Hericium erinaceus mycelium on lactic acid bacteria were also studied. Methods: Orthogonal design was used to study the effect on the total phenol content in liquid mycelium, and HPD-826 macroporous adsorption resin was used to separate it, and then HPLC-MS was used to determine its composition, which was added to the lactic acid bacteria culture medium to detect the number of viable bacteria and acid-producing ability of lactic acid bacteria. Results: When the carbon source potato was 14%, nitrogen source peptone was 2% and inorganic salt MgSO
4 was 0.3%, the total phenol content of
Hericium erinaceus mycelium reached 0.783 mg/g. Sixteen monomeric phenols were identified from the phenolic extract of
Hericium erinaceus mycelium, accounting for 88.27%. The main monomeric phenols were gallic acid -O- glucoside, p-coumaric acid -O- gallic acid glucoside, ferulic acid, catechin benzyl sulfide, gallic acid and caffeic acid. Adding phenol extraction solution from
Hericium erinaceus mycelium to MRS medium of
Lactobacilluscasei,
Lactobacillusacidophilus and
Streptococcus intestinalis can increase the number of viable bacteria of three kinds of lactic acid bacteria. Conclusion: Phenolic extract from
Hericium erinaceus mycelium can promote the growth of lactic acid bacteria and provide new resource raw materials for functional food.