响应面法优化超声辅助提取白兰叶总黄酮工艺
Ultrasonic- assisted extraction of total flavonoids from Michelia Alba leaves using response surface methodology
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摘要: 为优化超声波辅助提取白兰叶总黄酮工艺,在单因素实验的前提下,以总黄酮得率为响应值,选择乙醇浓度、液料比、超声时间、超声温度4个因素为自变量,进行Box-Behnken实验设计,研究4个因素及其交互作用对白兰叶总黄酮得率的影响。研究结果表明超声波辅助提取白兰叶总黄酮的最佳提取工艺条件为:乙醇浓度为54%,液料比为19 m L/g,超声时间为25 min,超声温度为66℃,在此条件下,测得白兰叶总黄酮的得率为9.94%,与预测值相对误差为0.30%,说明回归模型的拟合度较高,该研究为白兰叶开发成天然抗氧剂奠定了理论基础和科学依据。Abstract: The ultrasonic- assisted extraction conditions of polyphenol from michelia alba leaves were optimized by response surface methodology.Based on single factor test,a 4- factor,3- level Box- Behnken experimental was adopted including dependent variables( ethanol concentration,liquid to material ratio,ultrasonic time and ultrasonic temperature) and independent variable( yield of polyphenols). The results showed that the optimum conditions were ethanol concentration 54%,liquid to material ratio 19 m L / g,ultrasonic time 25 min,ultrasonic temperature66 ℃. The yield of total flavonoids could be up to 9.94% under the optimal extraction condition,the relative error was 0.30% compared to the predictive value,which indicated the feasible model fitted well with the experimental data.The research could be developed a theoretical foundation and a scientific basis for the extraction of total flavonoids from michelia alba leaves into natural antioxidant.